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pVenusCfN
(Plasmid #128566)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 128566 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST15
  • Backbone manufacturer
    ThermoFisher Scientific
  • Backbone size w/o insert (bp) 7470
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Venus
  • Alt name
    Split-GFP
  • Alt name
    Split Venus
  • Species
    Aequorea victoria
  • Insert Size (bp)
    474

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Tiago Fleming Outeiro; University Medical Center Göttingen Department of Experimental Neurodegeneration

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: AgeI (unknown if destroyed), 3' cloning site: MluI (unknown if destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVenusCfN was a gift from Oliver Vugrek (Addgene plasmid # 128566 ; http://n2t.net/addgene:128566 ; RRID:Addgene_128566)
  • For your References section:

    Combining Unique Multiplex Gateway Cloning and Bimolecular Fluorescence Complementation (BiFC) for High-Throughput Screening of Protein-Protein Interactions. Lepur A, Kovacevic L, Beluzic R, Vugrek O. J Biomol Screen. 2016 Dec;21(10):1100-1111. doi: 10.1177/1087057116659438. Epub 2016 Jul 28. 10.1177/1087057116659438 PubMed 27455993