pDONR_221_middle-entry_NanoLuc
(Plasmid
#128673)
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PurposeNanoLuc CDS middle entry gateway clone
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 128673 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR-221
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Backbone manufacturerThermofisher
- Backbone size w/o insert (bp) 4762
- Total vector size (bp) 3062
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Modifications to backboneBP recombination reaction with desired insert
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNanoLuc
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Insert Size (bp)523
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR_221_middle-entry_NanoLuc was a gift from Steven Farber (Addgene plasmid # 128673 ; http://n2t.net/addgene:128673 ; RRID:Addgene_128673) -
For your References section:
The LipoGlo reporter system for sensitive and specific monitoring of atherogenic lipoproteins. Thierer JH, Ekker SC, Farber SA. Nat Commun. 2019 Jul 31;10(1):3426. doi: 10.1038/s41467-019-11259-w. 10.1038/s41467-019-11259-w PubMed 31366908
