pCR2.1-KRAS-G12V
(Plasmid
#129091)
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PurposeKI KRAS G12V mutation into human KRAS locus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129091 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCR2.1-TOPO cloning vector
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Vector typehomologous recombination donor plasmid
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Selectable markersKRAS(G12V)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKRAS(G12V)
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SpeciesH. sapiens (human)
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MutationG12V
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Entrez GeneKRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RASK2, c-Ki-ras, c-Ki-ras2)
Cloning Information
- Cloning method Ligation Independent Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCR2.1-KRAS-G12V was a gift from Toshiro Sato (Addgene plasmid # 129091 ; http://n2t.net/addgene:129091 ; RRID:Addgene_129091) -
For your References section:
Modeling colorectal cancer using CRISPR-Cas9-mediated engineering of human intestinal organoids. Matano M, Date S, Shimokawa M, Takano A, Fujii M, Ohta Y, Watanabe T, Kanai T, Sato T. Nat Med. 2015 Mar;21(3):256-62. doi: 10.1038/nm.3802. Epub 2015 Feb 23. 10.1038/nm.3802 PubMed 25706875
