pinducer 20 DN-KASHΔPPPL
(Plasmid #129280)

• Purpose: Tet-ON inducible lentivirus expressing mcherry-labeled DN KASHΔPPPL
• Depositing Lab: Daniel Conway
• Publication: Zhang et al Curr Biol. 2019 Aug 2. pii: S0960-9822(19)30866-8. doi: 10.1016/j.cub.2019.07.021.

Backbone

• Vector backbone: pinducer20
• Backbone manufacturer: received from addgene, plasmid 44012
• Total vector size (bp): 11120
• Vector type: Lentiviral
• Selectable markers: Gentamicin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SYNE1
• Alt name: DN-KASHΔPPPL
• Species: H. sapiens (human)
• Insert Size (bp): 1000
• Mutation: the last 4 amino acids (PPPL) of the KASH domain were removed. This construct cannot interact with SUN1 or SUN2, making it a *defective* dominant negative, which is suitable as a control.
• GenBank ID: NM_182961.4
• Entrez Gene: SYNE1 (a.k.a. 8B, AMC3, AMCM, ARCA1, C6orf98, CPG2, EDMD4, KASH1, MYNE1, Nesp1, SCAR8, dJ45H2.2)
• Promoter: CMV
• Tag / Fusion Protein:
  • mcherry (N terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: LNCX

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry