p415 Glo3 S389,398 D
(Plasmid
#129486)
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PurposeExpression in S. cerevisiae
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 129486 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneP415 Met25
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGlo3 S389,398 D
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Alt nameYER122C
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SpeciesS. cerevisiae (budding yeast)
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Insert Size (bp)1482
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MutationMutation of serines 389 and 398 to Asp
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Entrez GeneGLO3 (a.k.a. YER122C)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
p415 Glo3 S389,398 D was a gift from Blanche Schwappach (Addgene plasmid # 129486 ; http://n2t.net/addgene:129486 ; RRID:Addgene_129486) -
For your References section:
Dissection of GTPase-activating proteins reveals functional asymmetry in the COPI coat of budding yeast. Arakel EC, Huranova M, Estrada AF, Rau EM, Spang A, Schwappach B. J Cell Sci. 2019 Aug 29;132(16). pii: jcs.232124. doi: 10.1242/jcs.232124. 10.1242/jcs.232124 PubMed 31331965
