pIRES2-hPAC-FLAG
(Plasmid #129494)

• Purpose: Expresses human TMEM206 tagged at the C-terminus with FLAG
• Depositing Lab: Zhaozhu Qiu
• Publication: Yang et al Science. 2019 Apr 26;364(6438):395-399. doi: 10.1126/science.aav9739. Epub 2019 Apr 25.

Backbone

• Vector backbone: pIRES2-EGFP
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 6377
• Modifications to backbone: Insert hPAC C-FLAG
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human PAC tagged with FLAG tag at the C-terminal end
• Alt name: C-terminus FLAG-tagged hPAC
• Alt name: hPAC C-FLAG
• Alt name: TMEM206
• Species: H. sapiens (human)
• Insert Size (bp): 1077
• GenBank ID: NP_060722
• Entrez Gene: PACC1 (a.k.a. C1orf75, PAC, TMEM206)
• Promoter: CMV
• Tag / Fusion Protein:
  • FLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CMV - F
• 3′ sequencing primer: IRES - Rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry