Addgene - pRK5-myc-Cdc42-Q61L Plasmid Data

Plasmid 12974: pRK5-myc-Cdc42-Q61L

Gene/insert name:
Cdc42 constitutively active
Alt name:
Cdc42
Insert size:
576
Species:
H. sapiens (human)
GenBank ID:
M57298
Entrez Gene:
CDC42 (RP1-224A6.5, CDC42Hs, G25K)
Fusion protein or tag:
Myc
Terminal:
N terminal on backbone
Mutation:
Q61L Constitutively active
Vector backbone:
pRK5-Myc
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
4750
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
SP6 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
A. Hall
Sequence: View sequences (1)
Principal Investigator:
Gary Bokoch
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 12974" in your Materials and Methods section.