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IRE1 alpha KA-pcDNA3.EGFP
(Plasmid #13010)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13010 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3-EGFP
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Inositol Requiring Enzyme-1
  • Alt name
    IRE1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3500
  • Mutation
    R4L, K599A
  • Entrez Gene
    ERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    IRE1 alpha KA-pcDNA3.EGFP was a gift from Fumihiko Urano (Addgene plasmid # 13010)
  • For your References section:

    Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1. Lipson KL, Fonseca SG, Ishigaki S, Nguyen LX, Foss E, Bortell R, Rossini AA, Urano F. Cell Metab. 2006 Sep . 4(3):245-54. 10.1016/j.cmet.2006.07.007 PubMed 16950141