Addgene - pcDNA3 V5 hHR23A Plasmid Data

Plasmid 13053: pcDNA3 V5 hHR23A

Gene/insert name:
hHR23A
Alt name:
HR23A
Alt name:
Rad23a
Insert size:
1100
Species:
H. sapiens (human)
Entrez Gene:
RAD23A (HHR23A, MGC111083)
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Fusion protein or tag:
His
Terminal:
C terminal on backbone
Vector backbone:
pcDNA3.1D V5 His TOPO
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5500
Cloning method:
TOPO Cloning
5' sequencing primer:
T7 List of Sequencing Primers
3' sequencing primer:
BGH rev
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (1)
Principal Investigator:
Steven Grossman
Terms and Licenses:
MTA

Comments:

You can cut out the hHR23A insert with HindIII and XhoI.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: A post-ubiquitination role for MDM2 and hHR23A in the p53 degradation pathway. Brignone et al (Oncogene. 2004 May 20. 23(23):4121-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13053" in your Materials and Methods section.