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40 p tie2
(Plasmid #13064)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 13064 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tie2
  • Species
    D. rerio (zebrafish)
  • Mutation
    Q817E (please see depositor's comment below)
  • Entrez Gene
    tek (a.k.a. tie-2, tie2)

Cloning Information

Resource Information

  • Terms and Licenses

Depositor Comments

For riboprobe, digest with Eco 01091 and use T3 polymerase. The depositor noted that the tie2 Q817R mutation found in Addgene's quality control sequence does NOT affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    40 p tie2 was a gift from Nathan Lawson (Addgene plasmid # 13064)
  • For your References section:

    Notch signaling is required for arterial-venous differentiation during embryonic vascular development. Lawson ND, Scheer N, Pham VN, Kim CH, Chitnis AB, Campos-Ortega JA, Weinstein BM. Development. 2001 Oct . 128(19):3675-83. PubMed 11585794