mTLR2
(Plasmid #13083)

• Depositing Lab: Ruslan Medzhitov
• Publication: toll-like receptors (unpublished)

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TLR2
• Alt name: toll-like receptor 2
• Species: M. musculus (mouse)
• Insert Size (bp): 2300
• GenBank ID:
• Entrez Gene: Tlr2 (a.k.a. Ly105)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: BGH rev

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that Addgene's sequencing results indicate that GenBank ID AAH14693.1 is better match for the mouse TLR2 insert sequence in this plasmid. When compared to GenBank ID NM_011905 there is an apparent mutation (M82I) which may represent a valid polymorphism.