pKIF1C-2xFLAG
(Plasmid #130979)

• Purpose: Expression of KIF1C-2xFLAG in mammalian cells.
• Depositing Lab: Anne Straube
• Publication: Theisen et al Dev Cell. 2012 Dec 11;23(6):1153-66. doi: 10.1016/j.devcel.2012.11.005.

Backbone

• Vector backbone: pEGFP
• Backbone manufacturer: Clontech
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: KIF1C-2xFLAG
• Alt name: KIF1C kinesin family member 1C
• Species: H. sapiens (human)
• Mutation: 4 silent mutations that make this construct resistant to RNAi oligo siKIF1C-1 also used in this paper. Nucleotides 649-663 (TCCCATGCCGTCTTT) have been replaced with TCGCACGCAGTCTTC.
• Entrez Gene: KIF1C (a.k.a. LTXS1, SATX2, SAX2, SPAX2, SPG58)
• Promoter: CMV
• Tag / Fusion Protein:
  • 2xFLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • addgene_id_130979_pKIF1C-2xFLAG.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry