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pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk
(Plasmid #131143)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 131143 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGE-attB-GMR
  • Backbone size w/o insert (bp) 6170
  • Total vector size (bp) 8304
  • Vector type
    Insect Expression, Cre/Lox ; attB recombination site
  • Selectable markers
    mini-White

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mNeonGreen::3XFLAG::Crk
  • Alt name
    Crk
  • Species
    D. melanogaster (fly)
  • Insert Size (bp)
    2183
  • Entrez Gene
    Crk (a.k.a. Dmel_CG1587, CG1587, CRK, D-CRK, D-Crk, DCrk, Dcrk, Dmel\CG1587, crk, dCRK, dCrk)
  • Tags / Fusion Proteins
    • mNeonGreen (N terminal on insert)
    • 3XFLAG (N terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gaattgccggctgtacactt
  • 3′ sequencing primer gcgacagagtgagagagcaa
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGE-attB-GMR_mNeonGreen::3XFLAG::dCrk was a gift from Mark Peifer (Addgene plasmid # 131143 ; http://n2t.net/addgene:131143 ; RRID:Addgene_131143)
  • For your References section:

    The Crk adapter protein is essential for Drosophila embryogenesis, where it regulates multiple actin-dependent morphogenic events. Spracklen AJ, Thornton-Kolbe EM, Bonner AN, Florea A, Compton PJ, Fernandez-Gonzalez R, Peifer M. Mol Biol Cell. 2019 Jul 18:mbcE19050302. doi: 10.1091/mbc.E19-05-0302. 10.1091/mbc.E19-05-0302 PubMed 31318326