pET28P_dCrk
(Plasmid #131145)

• Purpose: Expresses 6XHis::dCrk (based on crk isoform RA).
• Depositing Lab: Mark Peifer
• Publication: Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302. doi: 10.1091/mbc.E19-05-0302.

Backbone

• Vector backbone: pET28b
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5369
• Total vector size (bp): 6140
• Modifications to backbone: thrombin cleavage site swapped out for PreScission protease cleavage site
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Crk
• Species: D. melanogaster (fly)
• Insert Size (bp): 816
• Entrez Gene: Crk (a.k.a. Dmel_CG1587, CG1587, CRK, D-CRK, D-Crk, DCrk, Dcrk, Dmel\CG1587, crk, dCRK, dCrk)
• Tag / Fusion Protein:
  • 6XHis (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: TCC CGC GAA ATT AAT ACG AC
• 3′ sequencing primer: CCC CAA GGG GTT ATG CTA GT

Resource Information

• Supplemental Documents:
  • pET28P_dCrk.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry