Addgene - pc3XB-ZF54 Plasmid Data

Plasmid 13153: pc3XB-ZF54

Gene/insert name:
ZF54
Insert size:
90
Vector backbone:
pc3XB
(Search Vector Database)
Backbone manufacturer:
Modified Invitrogen pcDNA3
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Sequence: View sequences (2)
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

Part of the Zinc Finger Consortium Modular Assembly Kit v1.0. Finger protein sequence: PGEKKFACPECPKRFMRSDNLTEHIKTH. Target DNA sequence: CGG. This zinc finger is derived from the Sangamo module set.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13153" in your Materials and Methods section.