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pet 3d alpha tentacle delete CP
(Plasmid #13299)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 13299 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET- 3d
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    capping protein alpha 1 (deletion 28 amino acids) beta 1 subunits
  • Alt name
    actin CAP Z alpha tentacle delete
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2000
  • Entrez Gene
    CAPZB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nco 1 for alpha subunit (not destroyed)
  • 3′ cloning site bam H1 (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7-Term
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Soeno et al 1998; journal of muscle research and cell motility 19, 639-646

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet 3d alpha tentacle delete CP was a gift from John Cooper (Addgene plasmid # 13299)
  • For your References section:

    How capping protein binds the barbed end of the actin filament. Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA. Curr Biol. 2003 Sep 2. 13(17):1531-7. 10.1016/S0960-9822(03)00559-1 PubMed 12956956