pet 3d beta tentacle delete CP
(Plasmid #13300)

• Depositing Lab: John Cooper
• Publication: Wear et al Curr Biol. 2003 Sep 2. 13(17):1531-7.

Backbone

• Vector backbone: pET- 3d
• Backbone manufacturer: novagen
• Backbone size w/o insert (bp): 5000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: capping protein alpha 1 beta 1 (deletion 28 amino acids )
• Alt name: actin CAP Z beta tentacle delete
• Species: G. gallus (chicken)
• Insert Size (bp): 2000
• Mutation: CP alpha subunit is intact with a mutated beta C-termiinus (delete 28 amino acids)
• Entrez Gene: CAPZB

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: nde 1 (not destroyed)
• 3′ cloning site: bam H1 (not destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7-Term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Obinata T gave the plasmid for wt CP alpha 1 and beta 1 subunits. The mutant was generated on this background by PCR.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Soeno et al 1998; journal of muscle research and cell motility 19, 639-646