pet 3d beta tentacle delete CP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13300||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepET- 3d
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Gene/Insert namecapping protein alpha 1 beta 1 (deletion 28 amino acids )
Alt nameactin CAP Z beta tentacle delete
SpeciesG. gallus (chicken)
Insert Size (bp)2000
MutationCP alpha subunit is intact with a mutated beta C-termiinus (delete 28 amino acids)
- Cloning method Restriction Enzyme
- 5′ cloning site nde 1 (not destroyed)
- 3′ cloning site bam H1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7-Term (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byObinata T gave the plasmid for wt CP alpha 1 and beta 1 subunits. The mutant was generated on this background by PCR.
Terms and Licenses
Soeno et al 1998; journal of muscle research and cell motility 19, 639-646
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pet 3d beta tentacle delete CP was a gift from John Cooper (Addgene plasmid # 13300)
For your References section:How capping protein binds the barbed end of the actin filament. Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA. Curr Biol. 2003 Sep 2. 13(17):1531-7. 10.1016/S0960-9822(03)00559-1 PubMed 12956956