pet 3d double tentacle delete CP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13301||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepET- 3d
- Backbone size w/o insert (bp) 5000
Vector typeBacterial Expression
Growth in Bacteria
Gene/Insert namecapping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)
Alt nameactin CAP Z double tentacle delete
SpeciesG. gallus (chicken)
Insert Size (bp)2000
MutationCapping protein alpha 1 (with 28 amino acids deleted in the C-terminus) and beta 1 (with 34 amino acids deleted in the C-terminus)
- Cloning method Restriction Enzyme
- 5′ cloning site nco 1 for alpha subunit (not destroyed)
- 3′ cloning site bam H1 (not destroyed)
- 5′ sequencing primer SEQUENCE
- 3′ sequencing primer SEQUENCE (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byThe wt Capping protein plasmid was received from T. Obinata . PCR mutagenesis was done to delete the alpha and beta tentacles. Check Wear et al., 2003. for more information on the primers.
Terms and Licenses
Soeno et al., 1998
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pet 3d double tentacle delete CP was a gift from John Cooper (Addgene plasmid # 13301)
For your References section:How capping protein binds the barbed end of the actin filament. Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA. Curr Biol. 2003 Sep 2. 13(17):1531-7. 10.1016/S0960-9822(03)00559-1 PubMed 12956956