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pet 3d double tentacle delete CP
(Plasmid #13301)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 13301 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET- 3d
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    capping protein alpha 1 (deletion 28 amino acids) beta 1 (deletion 34 amino acids)
  • Alt name
    actin CAP Z double tentacle delete
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2000
  • Mutation
    Capping protein alpha 1 (with 28 amino acids deleted in the C-terminus) and beta 1 (with 34 amino acids deleted in the C-terminus)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nco 1 for alpha subunit (not destroyed)
  • 3′ cloning site bam H1 (not destroyed)
  • 5′ sequencing primer SEQUENCE
  • 3′ sequencing primer SEQUENCE
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The wt Capping protein plasmid was received from T. Obinata . PCR mutagenesis was done to delete the alpha and beta tentacles. Check Wear et al., 2003. for more information on the primers.
  • Terms and Licenses

Depositor Comments

Soeno et al., 1998

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet 3d double tentacle delete CP was a gift from John Cooper (Addgene plasmid # 13301)
  • For your References section:

    How capping protein binds the barbed end of the actin filament. Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA. Curr Biol. 2003 Sep 2. 13(17):1531-7. 10.1016/S0960-9822(03)00559-1 PubMed 12956956