Addgene - pLenti6-DEST PINK1-V5 WT Plasmid Data

Plasmid 13320: pLenti6-DEST PINK1-V5 WT

Gene/insert name:
PINK1
Insert size:
1743
Species:
H. sapiens (human)
Entrez Gene:
PINK1 (BRPK, PARK6)
Fusion protein or tag:
V5
Terminal:
C terminal on backbone
Vector backbone:
pLenti6/V5-DEST
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Lentiviral
Backbone size w/o insert (bp):
8688
Cloning method:
Gateway Cloning
Cloning site 5':
attR1
Site destroyed during cloning:
No
Cloning site 3':
attR2
Site destroyed during cloning:
No
5' sequencing primer:
CMV-F List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Blasticidin
Sequence: View sequences (3)
Principal Investigator:
Mark Cookson
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Mutations in PTEN-induced putative kinase 1 associated with recessive parkinsonism have differential effects on protein stability. Beilina et al (Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13320" in your Materials and Methods section.