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pGEX5X.1 PINK1 KD
(Plasmid #13322)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13322 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX5X.1
  • Backbone manufacturer
    Amersham
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PINK1 kinase domain
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1152
  • Mutation
    Amino acids 112-496; Kinase dead: K219D/D362A/D384A
  • Entrez Gene
    PINK1 (a.k.a. BRPK, PARK6)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEX5X.1 PINK1 KD was a gift from Mark Cookson (Addgene plasmid # 13322 ; http://n2t.net/addgene:13322 ; RRID:Addgene_13322)
  • For your References section:

    Mutations in PTEN-induced putative kinase 1 associated with recessive parkinsonism have differential effects on protein stability. Beilina A, Van Der Brug M, Ahmad R, Kesavapany S, Miller DW, Petsko GA, Cookson MR. Proc Natl Acad Sci U S A. 2005 Apr 19. 102(16):5703-8. 10.1073/pnas.0500617102 PubMed 15824318