pUC-GFP-AT
(Plasmid #133306)

• Purpose: "Burdensome" GFP expressing plasmid carrying axe/txe toxin-antitoxin for plasmid stabilisation
• Depositing Lab: Chris Barnes
• Publication: Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 10.1016/j.isci.2019.03.019. Epub 2019 Mar 22.

Backbone

• Vector backbone: pSF_UC_OXB20-daGFP
• Backbone manufacturer: Oxford Genetics
• Backbone size w/o insert (bp): 4700
• Total vector size (bp): 6475
• Modifications to backbone: original SC101 ori replaced with pUC ori
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: axe/txe toxin-antitoxin cassette
• Species: E. coli
• Insert Size (bp): 1998

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: SacI (unknown if destroyed)
• 3′ cloning site: XhoI (unknown if destroyed)
• 5′ sequencing primer: TCGTTGCGTTACACACAC
• 3′ sequencing primer: AGTCAGTCAGTGCAGGAG

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry