Addgene - pcDNA-GATA3-DC+Nzf Plasmid Data

Plasmid 1334: pcDNA-GATA3-DC+Nzf

Gene/insert name:
GATA3
Alt name:
GATA-3
Insert size:
1100
Species:
M. musculus (mouse)
GenBank ID:
NM_008091
Entrez Gene:
Gata3 (RP23-276D17.1, Gata-3, jal)
Vector backbone:
pcDNA3.1(+)
(Search Vector Database)
Backbone manufacturer:
Invitrogen
Vector type:
Mammalian Expression, Bacterial Expression
Backbone size w/o insert (bp):
5400
Cloning site 5':
EcoRV
Site destroyed during cloning:
No
Cloning site 3':
XbaI
Site destroyed during cloning:
No
5' sequencing primer:
T7 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Gokhan Hotamisligil
Terms and Licenses:
MTA

Comments:

Amino acids 257-341 in GATA-3 were deleted in the DC+Nzf mutant to remove both zinc fingers.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Function of GATA transcription factors in preadipocyte-adipocyte transition. Tong et al (Science 2000 Oct 6;290(5489):134-8. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1334" in your Materials and Methods section.