pst39-NST-clone14
(Plasmid #133421)

• Purpose: S. cerevisiae Nip1 (with C-terminal TEV cleavage site and strep-II tag), Sui1 and Tif5B6 (with N-terminal 7xHis tag and TEV cleavage set) coding sequences with intervening ribosome binding sites and other cis-elements for expression as a polycistron, T7 theta 10 promoter (and corresponding terminator after polycistron).
• Depositing Lab: Gerhard Wagner
• Publication: Wagner Lab plasmids (unpublished)

Backbone

• Vector backbone: pST39
• Backbone size w/o insert (bp): 2800
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NIP1/SUI1/TIF5
• Species: S. cerevisiae (budding yeast)
• Mutation: ORF for Nip1 encodes aa 4-156 (not complete ORF), ORF for Tif5 (B6) encodes aa 241-405 (not complete ORF)
• Entrez Gene: NIP1 (a.k.a. YMR309C)
• Entrez Gene: TIF5 (a.k.a. YPR041W, SUI5)
• Entrez Gene: SUI1 (a.k.a. YNL244C, MOF2, RFR1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HMS clone ID number: ScCD00061648.