pAV0714
(Plasmid
#133479)
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PurposeExpression in fission yeast from promoter pact1, integrated at ura4 locus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 133479 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepUra4(AfeI)(pAV0133)
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namepUra4(AfeI)-p(act1)-sfGFP-terminator(ScCYC1)
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SpeciesS. pombe (fission yeast)
Cloning Information
- Cloning method Unknown
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please see the Resource Information section for an annotated Genbank file.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAV0714 was a gift from Sophie Martin (Addgene plasmid # 133479 ; http://n2t.net/addgene:133479 ; RRID:Addgene_133479) -
For your References section:
A toolbox of stable integration vectors in the fission yeast Schizosaccharomyces pombe. Vjestica A, Marek M, Nkosi PJ, Merlini L, Liu G, Berard M, Billault-Chaumartin I, Martin SG. J Cell Sci. 2020 Jan 8;133(1). pii: jcs.240754. doi: 10.1242/jcs.240754. 10.1242/jcs.240754 PubMed 31801797
