Addgene - pUSti1 Plasmid Data

Plasmid 1335: pUSti1

Gene/insert name:
Sti1
Insert size:
2000
Species:
S. cerevisiae (budding yeast)
Entrez Gene:
STI1 (YOR027W)
Vector backbone:
pUGPD (pRS316 carrying GPD promoter, based on pBluescript)
(Search Vector Database)
Vector type:
Yeast Expression
Backbone size w/o insert (bp):
5000
5' sequencing primer:
n/a List of Sequencing Primers
3' sequencing primer:
n/a
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
Low Copy
Sequence: View sequences (2)
Principal Investigator:
Susan Lindquist
Terms and Licenses:
MTA

Comments:

Plasmid maintained in DH5alpha, CEN and selectable URA3 marker. Full-length Sti1 gene, expressed at level comparable to the endogenous gene.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: In vivo analysis of the Hsp90 cochaperone Sti1 (p60). Chang et al (Mol Cell Biol 1997 Jan;17(1):318-25. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 1335" in your Materials and Methods section.