ps226
(Plasmid #133557)

• Purpose: RNAi construct for knock-down of CDKN1A (Cip1, p21)
• Depositing Lab: David Livingston
• Publication: Chan et al Genes Dev. 2005 Jan 15;19(2):196-201. doi: 10.1101/gad.1280205.

Backbone

• Vector backbone: pRETRO-SUPER
• Backbone size w/o insert (bp): 6349
• Vector type: Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CDKN1A
• gRNA/shRNA sequence: gaccatgtggacctgtcac
• Species: H. sapiens (human)
• Entrez Gene: CDKN1A (a.k.a. CAP20, CDKN1, CIP1, MDA-6, P21, SDI1, WAF1, p21CIP1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

HMS clone ID number: HsSH00021241.

Ho Man Chan made the clone.

According to the clone donor, NOT identical to the shRNA reported in the paper (that is ps225) but this is a functional shRNA against ps226.