|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||13371||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert namecatenin (cadherin associated protein), beta 1 (S33Y mutant)
Alt namebeta-catenin S33Y, L388P
SpeciesM. musculus (mouse)
Insert Size (bp)2346
MutationSerine 33 to Tyrosine and Leucine 388 to Proline
Entrez GeneCtnnb1 (a.k.a. Bfc, Catnb, Mesc)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pMX-S1811 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypMXs is from Dr. Toshio Kitamura of the University of Tokyo, the Institute of Medical Science. If you use this plasmid in a paper, please cite: Retrovirus-mediated gene transfer and expression cloning: powerful tools in functional genomics. Exp Hematol. 2003 Nov;31(11):1007-14. Kitamura T, Koshino Y, Shibata F, Oki T, Nakajima H, Nosaka T, Kumagai H.
Terms and Licenses
The sequence of primer pMX-S1811 is GAC GGC ATC GCA GCT TGG ATA CAC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pMXs-beta-catenin-S33Y was a gift from Shinya Yamanaka (Addgene plasmid # 13371)
For your References section:Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Takahashi K, Yamanaka S. Cell. 2006 Aug 25. 126(4):663-76. 10.1016/j.cell.2006.07.024 PubMed 16904174