pCas3cRh
(Plasmid #133773)

• Purpose: Expresses all necessary components of Type I-C CRISPR-Cas system
• Depositing Lab: Joseph Bondy-Denomy
• Publication: Csorgo et al Nat Methods. 2020 Oct 19. pii: 10.1038/s41592-020-00980-w. doi: 10.1038/s41592-020-00980-w.

Backbone

• Vector backbone: pHERD30T
• Backbone manufacturer: Dongru Qiu, Hongwei D. Yu
• Backbone size w/o insert (bp): 5216
• Total vector size (bp): 11502
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Gentamicin, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: RhaR
• Insert Size (bp): 849
• Entrez Gene: rhaR (a.k.a. b3906, ECK3899, rhaC1)

Gene/Insert 2

• Gene/Insert name: RhaS
• Insert Size (bp): 837
• Entrez Gene: rhaS (a.k.a. b3905, ECK3898, rhaC2)

Gene/Insert 3

• Gene/Insert name: pRhaBAD
• Insert Size (bp): 36

Gene/Insert 4

• Gene/Insert name: I-C crRNA
• Insert Size (bp): 88

Gene/Insert 5

• Gene/Insert name: Cas3
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 2235

Gene/Insert 6

• Gene/Insert name: Cas5
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 654

Gene/Insert 7

• Gene/Insert name: Cas8
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 1764

Gene/Insert 8

• Gene/Insert name: Cas7
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 870

Resource Information

• Supplemental Documents:
  • p30t-rha-ic-crrna_bsai-1.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note: Plasmid contains a K244R mutation in pRO1600 Rep. This mutation is not known to affect plasmid function.