Addgene - pGEX-4T-2 RanGAP Plasmid Data

Plasmid 13379: pGEX-4T-2 RanGAP

Gene/insert name:
RanGAP
Alt name:
RanGAP1
Insert size:
1800
Species:
H. sapiens (human)
Entrez Gene:
RANGAP1 (RP4-756G23.7, Fug1, SD)
Fusion protein or tag:
GST
Terminal:
N terminal on backbone
Vector backbone:
pGEX-4T-2
(Search Vector Database)
Backbone manufacturer:
Amersham
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
5000
Cloning site 5':
SmaI
Site destroyed during cloning:
No
Cloning site 3':
SmaI
Site destroyed during cloning:
No
5' sequencing primer:
pGEX 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (1)
Principal Investigator:
Mary Dasso
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: SUMO-1 targets RanGAP1 to kinetochores and mitotic spindles. Joseph et al (J Cell Biol. 2002 Feb 18. 156(4):595-602. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13379" in your Materials and Methods section.