pFR092
(Plasmid
#134148)
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PurposeExpression of CENP-T
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134148 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET3aTr
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Backbone manufacturerSong Tan (Addgene plasmid # 64008)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCENP-T
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SpeciesH. sapiens (human)
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Mutationaa 1-375
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Entrez GeneCENPT (a.k.a. C16orf56, CENP-T, SSMGA)
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Tag
/ Fusion Protein
- His (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site BamHI (destroyed during cloning)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFR092 was a gift from Iain Cheeseman (Addgene plasmid # 134148 ; http://n2t.net/addgene:134148 ; RRID:Addgene_134148) -
For your References section:
CENP-T provides a structural platform for outer kinetochore assembly. Nishino T, Rago F, Hori T, Tomii K, Cheeseman IM, Fukagawa T. EMBO J. 2013 Feb 6;32(3):424-36. doi: 10.1038/emboj.2012.348. Epub 2013 Jan 18. 10.1038/emboj.2012.348 PubMed 23334297
