Addgene - pGP-FB-orig BA Plasmid Data

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Plasmid 13420: pGP-FB-orig BA

Gene/insert name:		orig BA
Alternative names:		original BCR-ABL three-finger array
Insert size (bp):		260
Species of gene(s):		Other
Fusion proteins or tags:		Gal11P
Terminal:		N terminal on backbone
Vector backbone:		pGP-FB (Search Vector Database)
Type of vector:		Bacterial expression
Backbone size (bp):		3483
Cloning site 5':		XbaI
Site destroyed during cloning:		No
Cloning site 3':		BamHI
Site destroyed during cloning:		No
5' Sequencing primer:		GGG GGA TTT CTG TTC ATG GGG G (List of Sequencing Primers)
Bacteria resistance:		Ampicillin
High or low copy:		Low Copy
Grow in standard E. coli @ 37C:		No
Please specify bacterial strain for growth and growth condition:		Needs strain with lacIq
Sequence:		View sequence
Author's Map:		View map
Plasmid Provided In:		XL1 Blue
Principal Investigator:		Keith Joung
Terms and Licenses:		MTA

Comments: Positive control for B2H beta-galactosidase assay. Has coding sequence for �original� BCR-ABL three-finger array.

This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter, which directs expression of the zinc finger-Gal11P hybrid protein.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

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Selected features

lac_promoter

12 - 35

M13_pUC_rev_primer

55 - 77

f1_origin

810 - 1116

AmpR_promoter

1309 - 1337

Orf frame 2

1379 - 2239

Ampicillin

1379 - 2239

pBR322_origin

2394 - 3013

pGEX_3_primer

3410 - 3432

ROP

3617 - 3426

Unique restriction sites

EcoRI		1
SalI		44
HindIII		351
NotI		360
EagI		360
XbaI		392
AgeI		482
PstI		630
BamHI		656
AseI		1991
NdeI		3233
PvuII		3465

Please acknowledge the principal investigator if you use this plasmid in a publication.

Also, please include the text "Addgene plasmid 13420" in your Materials and Methods section. This information allows Addgene to create a link from the plasmid page to your publication.