Addgene - pGP-FB-orig BA Plasmid Data

Plasmid 13420: pGP-FB-orig BA

Gene/insert name:
orig BA
Alt name:
original BCR-ABL three-finger array
Insert size:
260
Fusion protein or tag:
Gal11P
Terminal:
N terminal on backbone
Vector backbone:
pGP-FB
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
3483
Cloning site 5':
XbaI
Site destroyed during cloning:
No
Cloning site 3':
BamHI
Site destroyed during cloning:
No
5' sequencing primer:
GGG GGA TTT CTG TTC ATG GGG G List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
XL1 Blue
Growth temperature (℃):
37
Growth instructions:
Needs strain with lacIq
High or low copy:
Low Copy
Sequence: View sequences (2)
Map:
View map
Principal Investigator:
Keith Joung
Terms and Licenses:
MTA

Comments:

Positive control for B2H beta-galactosidase assay. Has coding sequence for �original� BCR-ABL three-finger array.

This plasmid requires a bacterial strain, such as XL1 Blue, that expresses the lacIq allele of lac repressor. This ensures repression of the strong lacUV5 promoter, which directs expression of the zinc finger-Gal11P hybrid protein.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13420" in your Materials and Methods section.