Addgene - U6 pShuttle Plasmid Data

Gene/insert name:
None
Vector backbone:
pShuttle
(Search Vector Database)
Vector type:
Mammalian Expression, Adenoviral
Modifications to Backbone:
human U6 promoter inserted into pShuttle
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance:
Kanamycin
Growth strain:
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Person or lab that originally
cloned the gene/insert:
The U6 promoter was cloned from Imgenex's plasmid.
Sequence: View sequences (1)
Principal Investigator:
Ronald Kahn
Terms and Licenses:
MTA

Comments:

pShuttle is used to make adenovirus in the AdEasy system. The human U6 promoter allows for siRNA expression.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13428" in your Materials and Methods section.