pB6-Lrig1-T2A-tdTomato-FNF-TK
(Plasmid #134320)

• Purpose: B6J Lrig1-T2A-tdTomato allele targeting vector, low copy number
• Depositing Lab: Mario Capecchi
• Publication: Nam et al Neural Dev. 2020 Mar 17;15(1):3. doi: 10.1186/s13064-020-00139-5.

Backbone

• Vector backbone: pWS-TK6
• Backbone manufacturer: Sen Wu, Capecchi laboratory
• Vector type: Mouse Targeting

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Lrig1
• Species: M. musculus (mouse)
• Tag / Fusion Protein:
  • C-terminal fusion of T2A-tdTomato

Resource Information

• Supplemental Documents:
  • pLrig1-T2A-tdTomato-FNF-TK fixed.gb
• A portion of this plasmid was derived from a plasmid made by: tdTomato is from Roger Tsien. FRT-Neo-FRT is from pFNF (#22687).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Homology arms subcloned from a clone of RPCI-24 C57BL/6J mouse genomic DNA library.

Please make sure the plasmid DNA preparation is pure by gel electrophoresis before linearizing for an electroporation.

Mice available from The Jackson Laboratory (https://www.jax.org/strain/036457).