PGKneotpAlox2
(Plasmid #13444)

• Depositing Lab: Philippe Soriano
• Publication: Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Backbone

• Vector backbone: na
• Backbone size w/o insert (bp): 2900
• Vector type: Mammalian Expression, Cre/Lox
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PGKneotpAlox2
• Alt name: PGKneotpA
• Insert Size (bp): 2800

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: See map (not destroyed)
• 3′ cloning site: See map (not destroyed)
• 5′ sequencing primer: na

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This vector contains: phosphoglycerate kinase I (PGK) promoter, neomycin resistance gene (neo), PGK polyA site, and 3x SV40 polyA site (tpA). The PGKneotpA is flanked by loxP sites.