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pet 3d alpha 1 beta 2 CP
(Plasmid #13450)


Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 13450 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
    pET- 3d
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number


  • Gene/Insert name
    capping protein alpha 1 beta 2 (mouse)
  • Alt name
    actin CP mouse
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
  • Entrez Gene
    Capza1 (a.k.a. CAPZ, CAZ1, Cappa1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nco 1 for alpha subunit. nd1 1 for beta (not destroyed)
  • 3′ cloning site bam H1 (not destroyed)
  • 5′ sequencing primer SEQUENCE
  • 3′ sequencing primer SEQUENCE
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pet 3d alpha 1 beta 2 CP was a gift from John Cooper (Addgene plasmid # 13450)
  • For your References section:

    How capping protein binds the barbed end of the actin filament. Wear MA, Yamashita A, Kim K, Maeda Y, Cooper JA. Curr Biol. 2003 Sep 2. 13(17):1531-7. 10.1016/S0960-9822(03)00559-1 PubMed 12956956