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CAP Z alpha 1 beta 1 CP
(Plasmid #13451)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 13451 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET- 3d
  • Backbone manufacturer
    novagen
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    capping protein alpha 1 beta 1 (chicken)
  • Alt name
    actin CAP Z
  • Alt name
    CAPZB
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    2000
  • Mutation
    none
  • Entrez Gene
    CAPZB

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site nco 1 for alpha subunit. nd1 1 for beta (not destroyed)
  • 3′ cloning site bam H1 (not destroyed)
  • 5′ sequencing primer SEQUENCE
  • 3′ sequencing primer SEQUENCE
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAP Z alpha 1 beta 1 CP was a gift from John Cooper (Addgene plasmid # 13451)
  • For your References section:

    Generation of functional beta-actinin (CapZ) in an E. coli expression system. Soeno Y, Abe H, Kimura S, Maruyama K, Obinata T. J Muscle Res Cell Motil. 1998 Aug . 19(6):639-46. 10.1023/A:1005329114263 PubMed 9742448