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pCAG-HA-Sox2-IP
(Plasmid #13459)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13459 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCAG-HA-gw-IP
  • Backbone size w/o insert (bp) 6800
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Sox2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    950
  • Entrez Gene
    Sox2 (a.k.a. Sox-2, lcc, ysb)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer pCAG-F (5'-GCAACGTGCTGGTTATTGTG-3')
  • 3′ sequencing primer IRES-R (5'-GCATTCCTTTGGCGAGAG-3')
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
  • Terms and Licenses
  • Articles Citing this Plasmid

Depositor Comments

The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-HA-Sox2-IP was a gift from Shinya Yamanaka (Addgene plasmid # 13459)
  • For your References section:

    Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells. Maruyama M, Ichisaka T, Nakagawa M, Yamanaka S. J Biol Chem. 2005 Jul 1. 280(26):24371-9. 10.1074/jbc.M501423200 PubMed 15863505