Addgene - pCAG-HA-Sox2-IP Plasmid Data

Plasmid 13459: pCAG-HA-Sox2-IP

Gene/insert name:
Sox2
Insert size:
950
Species:
M. musculus (mouse)
Entrez Gene:
Sox2 (AL606746.1, Sox-2, lcc, ysb)
Fusion protein or tag:
HA
Terminal:
N terminal on backbone
Mutation:
None
Vector backbone:
pCAG-HA-gw-IP
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert (bp):
6800
5' sequencing primer:
CAG-1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Person or lab that originally
cloned the gene/insert:
CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Comments:

The Sox2 cDNA contains a modified ATG start codon to TTG for cloning into vectors with an N-terminal tag.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Differential roles for Sox15 and Sox2 in transcriptional control in mouse embryonic stem cells. Maruyama et al (J Biol Chem. 2005 Jul 1. 280(26):24371-9. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13459" in your Materials and Methods section.