Addgene - pCAG-Oct3/4-IRES-Neo Plasmid Data

Gene/insert name:
Oct3/4
Insert size:
1150
Species:
M. musculus (mouse)
Entrez Gene:
Pou5f1 (NF-A3, Oct-3, Oct-3/4, Oct-4, Oct3, Oct3/4, Oct4, Otf-3, Otf-4, Otf3, Otf3-rs7, Otf3g, Otf4)
Mutation:
None
Vector backbone:
pCAG-gw-IRES-Neo
(Search Vector Database)
Vector type:
Mammalian Expression
Backbone size w/o insert:
6300
5' sequencing primer:
CAG-1 List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Neomycin
Person or lab that originally
cloned the gene/insert:
CAG promoter was from Dr. Jun-ichi Miyazaki of Osaka University Graduate School of Medicine. In publication using this plasmid, please cite: Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene 108:193-200, 1991. Niwa, H., Yamamura, K. & Miyazaki, J.
Sequence: View sequences (1)
Map:
View map
Principal Investigator:
Shinya Yamanaka
Terms and Licenses:
MTA

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Fbx15 is a novel target of Oct3/4 but is dispensable for embryonic stem cell self-renewal and mouse development. Tokuzawa et al (Mol Cell Biol. 2003 Apr . 23(8):2699-708. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13461" in your Materials and Methods section.