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pIRES-HMGA1
(Plasmid #13466)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13466 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pIRESpuro3
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 5100
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HMGA1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    323
  • GenBank ID
    NM_145899.2
  • Entrez Gene
    HMGA1 (a.k.a. HMG-R, HMGA1A, HMGIY)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRV (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IMAGE Consortium
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The HMGA1 coding sequence was PCR amplified from IMAGE clone 5399570 (Genbank accession no. BC063434) using gene-specific primers modified to include the appropriate restriction sites at their 5' end. The primers used were forward, 5'-TTTTGATATCATGAGTGAGTCGAGCTCGAAG-3' and backward, 5'-TTTTGAATTCTCACTGCTCCTCCTCCGAGGA-3'. Purified PCR products were digested with EcoRV and EcoRI before ligation into an EcoRV/EcoRI–digested pIRES-puro3 vector (Clontech, Palo Alto, CA).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-HMGA1 was a gift from Edward Whang (Addgene plasmid # 13466 ; http://n2t.net/addgene:13466 ; RRID:Addgene_13466)
  • For your References section:

    HMGA1 is a determinant of cellular invasiveness and in vivo metastatic potential in pancreatic adenocarcinoma. Liau SS, Jazag A, Whang EE. Cancer Res. 2006 Dec 15. 66(24):11613-22. 10.1158/0008-5472.CAN-06-1460 PubMed 17178855