pGL2-Basic-Myogenin core promoter
(Plasmid #134722)

• Purpose: Myogenin core promoter in a luciferase reporter plasmid
• Depositing Lab: Michael Chin
• Publication: Sun et al J Biol Chem. 2001 May 25;276(21):18591-6. doi: 10.1074/jbc.M101163200. Epub 2001 Feb 22.

Backbone

• Vector backbone: pGL2-Basic
• Backbone manufacturer: Promega
• Backbone size w/o insert (bp): 5597
• Total vector size (bp): 5781
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Myogenin Promoter
• Species: H. sapiens (human), M. musculus (mouse)
• Insert Size (bp): 184
• Promoter: Myogenin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (unknown if destroyed)
• 3′ cloning site: HindIII (unknown if destroyed)
• 5′ sequencing primer: CTCGAGCCTGCAGGGTGGGGTGGGGG
• 3′ sequencing primer: AAGCTTCCCCCAAGCTCCCGCAGCCC

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Eric Olsen's lab and obtained through the permission of Jon Epstein. Edmondson, D. G., Cheng, T. C., Cserjesi, P., Chakraborty, T., and Olson, E. N. (1992) Mol. Cell. Biol. 12, 3665–3677

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry