Addgene - mSin1 shRNA #2 Plasmid Data

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Plasmid 13484: mSin1 shRNA #2

Gene/insert name:
mSin1
Alt name:
shRNA mSin1
Alt name:
MAPKAP1
Insert size:
60
Species:
H. sapiens (human)
GenBank ID:
NM_024117
Entrez Gene:
MAPKAP1 (MIP1, SIN1, JC310, SIN1b, SIN1g, MGC2745)
Vector backbone:
pLKO.1
(Search Vector Database)
Vector type:
Lentiviral, RNAi
Backbone size w/o insert:
7032
Cloning site 5':
AgeI
Site destroyed during cloning:
Yes
Cloning site 3':
EcoRI
Site destroyed during cloning:
No
5' sequencing primer:
LKO.1 5' List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
Stbl3
Growth temperature (℃):
37
High or low copy:
High Copy
Selectable markers:
Puromycin
Sequence: View sequences (1)
Supplemental document:
Addgene's pLKO.1 protocol
Principal Investigator:
David Sabatini
Terms and Licenses:
MTA

Comments:

Short hairpin construct specific for human mSin1 (mSin1.1#2, TRC shRNA NM_024117.x-1308s1c1) from the RNAi Consortium. The target sequence is 5'-CCACA GTACA GGATA TGCTT-3'.

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: mSin1 Is Necessary for Akt/PKB Phosphorylation, and Its Isoforms Define Three Distinct mTORC2s. Frias et al (Curr Biol. 2006 Aug 15. ():. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13484" in your Materials and Methods section.