FpG5.BFP.Reporter
(Plasmid
#134967)
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Purpose(Empty Backbone) Lentiviral vector for enhancer reporter assay
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 134967 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLentiCRISPR-tagBFP
- Backbone size (bp) 7076
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Modifications to backboneThe lentiviral backbone is derived from pLentiCRISPR-tagBFP (Addgene #75160) and modified for enhancer reporter assay
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Vector typeMammalian Expression, Lentiviral
- Promoter mini Fgf4 promoter
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
The mini Fgf4 promoter is from FpG5 (Addgene #69443).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
FpG5.BFP.Reporter was a gift from Beat Bornhauser (Addgene plasmid # 134967 ; http://n2t.net/addgene:134967 ; RRID:Addgene_134967) -
For your References section:
The Leukemogenic TCF3-HLF Complex Rewires Enhancers Driving Cellular Identity and Self-Renewal Conferring EP300 Vulnerability. Huang Y, Mouttet B, Warnatz HJ, Risch T, Rietmann F, Frommelt F, Ngo QA, Dobay MP, Marovca B, Jenni S, Tsai YC, Matzk S, Amstislavskiy V, Schrappe M, Stanulla M, Gstaiger M, Bornhauser B, Yaspo ML, Bourquin JP. Cancer Cell. 2019 Dec 9;36(6):630-644.e9. doi: 10.1016/j.ccell.2019.10.004. Epub 2019 Nov 14. 10.1016/j.ccell.2019.10.004 PubMed 31735627
Map uploaded by the depositor.
