This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pTK164
(Plasmid #13533)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 13533 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FLIPW-CTY
  • Alt name
    ECFP-TrpR-Venus
  • Alt name
    TrpR
  • Species
    E. coli
  • Insert Size (bp)
    2000
  • Tags / Fusion Proteins
    • ECFP (N terminal on insert)
    • Venus (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

For more information, see
http://carnegiedpb.stanford.edu/research/frommer/nanosensors/index.html

TrpR was gateway cloned into FLIP cassette.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTK164 was a gift from Wolf Frommer (Addgene plasmid # 13533 ; http://n2t.net/addgene:13533 ; RRID:Addgene_13533)
  • For your References section:

    Nanosensor detection of an immunoregulatory tryptophan influx/kynurenine efflux cycle. Kaper T, Looger LL, Takanaga H, Platten M, Steinman L, Frommer WB. PLoS Biol. 2007 Oct;5(10):e257. 10.1371/journal.pbio.0050257 PubMed 17896864