pET26b_Nb.AT110
(Plasmid
#135480)
-
PurposeE. coli periplasmic expression of conformational nanobody Nb.AT110 recognizing angiotensin II type 1 receptor
-
Depositing Lab
-
Sequence Information
-
Sequences (2) — Accept Affinity Reagent Sequence Policy
-
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepET26b
-
Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 5680
-
Modifications to backbonen/a
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameNanobody AT110
-
Alt nameNb.AT110
-
SpeciesSynthetic
-
Insert Size (bp)949
- Promoter T7
-
Tags
/ Fusion Proteins
- pelB leader (N terminal on backbone)
- 6xHis-tag (C terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 forward
- 3′ sequencing primer T7 reverse (Common Sequencing Primers)
Resource Information
-
Addgene Notes
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pET26b_Nb.AT110 was a gift from Andrew Kruse (Addgene plasmid # 135480 ; http://n2t.net/addgene:135480 ; RRID:Addgene_135480) -
For your References section:
Distinctive Activation Mechanism for Angiotensin Receptor Revealed by a Synthetic Nanobody. Wingler LM, McMahon C, Staus DP, Lefkowitz RJ, Kruse AC. Cell. 2019 Jan 24;176(3):479-490.e12. doi: 10.1016/j.cell.2018.12.006. Epub 2019 Jan 10. 10.1016/j.cell.2018.12.006 PubMed 30639100
