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Purpose(Empty Backbone) High eukaryotic protein expression shuttle vector
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 135583 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepFastBac1
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Backbone manufacturerInvitrogen
- Backbone size (bp) 4736
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Modifications to backboneAn 80 bp cis-element inserted upstream of the polyhedrin promotor of AcMNPV to improve protein expression yields.
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Vector typeInsect Expression ; transfer vector
- Promoter polyhedrin of AcMNPV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac-M2 was a gift from Xiao-Wen Cheng (Addgene plasmid # 135583 ; http://n2t.net/addgene:135583 ; RRID:Addgene_135583) -
For your References section:
Improved pFastBac donor plasmid vectors for higher protein production using the Bac-to-Bac(R) baculovirus expression vector system. Shang H, Garretson TA, Kumar CMS, Dieter RF, Cheng XW. J Biotechnol. 2017 Aug 10;255:37-46. doi: 10.1016/j.jbiotec.2017.06.397. Epub 2017 Jun 20. 10.1016/j.jbiotec.2017.06.397 PubMed 28645582
Map uploaded by the depositor.
