Addgene - pRSET FLIPglu-2uDelta13 Plasmid Data

Plasmid 13561: pRSET FLIPglu-2uDelta13

Gene/insert name:
FLIPglu F16F D152A Delta13
Alt name:
ECFP-mglB-EYFP
Alt name:
Periplasmic glucose binding
Insert size:
2400
Species:
E. coli
Fusion protein or tag:
ECFP
Terminal:
N terminal on insert
Fusion protein or tag:
EYFP
Terminal:
C terminal on insert
Mutation:
F16A, D152A in binding protein
Vector backbone:
pRSET-B
(Search Vector Database)
Vector type:
Bacterial Expression
Backbone size w/o insert (bp):
2897
Cloning site 5':
BamHI
Site destroyed during cloning:
No
Cloning site 3':
HindIII
Site destroyed during cloning:
No
5' sequencing primer:
N/A List of Sequencing Primers
Bacterial resistance(s)
Ampicillin
Growth strain(s)
DH5alpha
Growth temperature (℃):
37
High or low copy:
High Copy
Sequence: View sequences (2)
Principal Investigator:
Wolf Frommer
Terms and Licenses:
MTA

Comments:

For more information, see
http://carnegiedpb.stanford.edu/research/fromme...

Addgene has sequenced a portion of this plasmid for verification. Click here for the sequencing result.

Article: Construction and optimization of a family of genetically encoded metabolite sensors by semirational protein engineering. Deuschle et al (Protein Sci. 2005 Sep . 14(9):2304-14. PubMed)

Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication. Also, please include the text "Addgene plasmid 13561" in your Materials and Methods section.