pTAHR TH-p2a-TD:Tomato (floxed selection Puro)
(Plasmid #135814)

• Purpose: Fluorescent reporter for knock-in of TdTomato into the TH locus
• Depositing Labs: Tim Ahfeldt, Lee Rubin
• Publication: Ahfeldt et al Stem Cell Reports. 2019 Dec 20. pii: S2213-6711(19)30444-8. doi: 10.1016/j.stemcr.2019.12.005.

Backbone

• Vector backbone: HR120PA-1
• Backbone manufacturer: SBI
• Backbone size w/o insert (bp): 7672
• Total vector size (bp): 9204
• Modifications to backbone: removed GFP fluorescent cassette inserted p2aTdTomato WPRE cassette via assembly, added 5'TH and 3'TH homology arms via assembly
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: TH-p2a-TD:Tomato (red fluorophore protein) construct as a reporter for TH expression.
• Alt name: last exon of human TH gene
• Species: H. sapiens (human)
• GenBank ID: NM_000360.4
• Entrez Gene: TH (a.k.a. DYT14, DYT5b, TYH)
• Tag / Fusion Protein:
  • p2a-TdTomato

Resource Information

• Supplemental Documents:
  • pTAHR TH-p2a-TD_Tomato (floxed selection Puro).gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry