pSLCAR-CD19-CD3z
(Plasmid #135993)

• Purpose: Modular CD3z alone CAR backbone, For Transient Expression or Lentiviral Production
• Depositing Lab: Scott McComb
• Publication: Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;16:238-254. doi: 10.1016/j.omtm.2020.01.012. eCollection 2020 Mar 13.

Backbone

• Vector backbone: pSL
• Backbone manufacturer: Heavily modified from LentiCRISPR v1 backbone
• Modifications to backbone: Use BpiI single pot restriction ligation to swap the antigen binding domain of the CAR from CD19 to desired specificity
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: FMC63-3z-P2A-EGFP
• Alt name: CD19(FMC63)-CAR, no costim., CD3z stim domains, P2A-GFP marker
• Alt name: Contains: FMC63-scFV, Hinge domain, CD28-transmembrane domain, CD3z-ITD
• Species: H. sapiens (human)
• Entrez Gene: CD247 (a.k.a. CD3-ZETA, CD3H, CD3Q, CD3Z, CD3ZETA, IMD25, T3Z, TCRZ)
• Entrez Gene: CD28 (a.k.a. Tp44)
• Promoter: EF1a-short

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BpiI (destroyed during cloning)
• 3′ cloning site: BpiI (destroyed during cloning)
• 5′ sequencing primer: CGGGTTTGCCGCCA
• 3′ sequencing primer: caggagccTGCTCCTCTTACTcc

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry