pDONR-U6gRNA-PGKpuro2APDGFB
(Plasmid #136409)

• Purpose: (Empty Backbone) Donor vector for gRNA subcloning. Required for gRNA cloning into the RCAS-DV. Also constitutively expresses PDGFB.
• Depositing Lab: Massimo Squatrito
• Publication: Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi: 10.1038/s41467-018-03731-w.

Backbone

• Vector backbone: pDONR-U6gRNA-PGKpuro2ABFP
• Modifications to backbone: BbsI restriction site in the PDGFB sequence (Glu173Asp174) was removed by site directed mutagenesis. HA tag on vector.
• Vector type: CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: None
• Mutation: BbsI restriction site in the PDGFB sequence (Glu173Asp174) was removed by site directed mutagenesis

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: Unknown

Resource Information

• Supplemental Documents:
  • pDONR-U6gRNA-PGKpuro2APDGFB.gb
• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry